ABSTRACT
Resumen Comunicamos la primera identificación en Ecuador del microorganismo Comamonas kerstersii, cepa aislada a partir de una muestra de líquido de la cavidad abdominal e identificada mediante la técnica de espectrometría de masas MALDITOF. Fue obtenida durante el acto quirúrgico de un paciente con el diagnóstico de una peritonitis aguda, secundaria a una apendicitis perforada. Este microorganismo es considerado un patógeno poco común. Pese a que el género al que pertenece tiene una amplia distribución geográfica, es considerado un agente oportunista.
Abstract We report the first identification in Ecuador of the microorganism Comamonas kerstersii, a strain isolates from a sample of fluid from the abdominal cavity and identified by the technique of mass spectrometry MALDITOF. It was obtained during the surgical act of a patient with the diagnosis of acute peritonitis, secondary to a perforated appendicitis. This microorganism is considered a rare pathogen. Even though the genus to which it belongs has a wide geographical distribution, this pathogen is considered an opportunistic agent.
Subject(s)
Humans , Comamonas , Peritonitis , EcuadorABSTRACT
Resumen Comamonas kerstersii es un bacilo gramnegativo no fermentador, asociado a infecciones intraabdominales. Comunicamos un caso clínico de una bacteriemia por C. kerstersii en un joven con una apendicitis aguda. La primera identificación, mediante el sistema Vitek2 compact (BioMerieux) desde los hemocultivos, fue Comamonas testosteroni. Sin embargo, mediante MALDI-TOF se identificó como C. kerstersii. El paciente fue tratado con ampicilina/sulbactam y luego piperacilina/tazobactam con una evolución favorable. Dentro del género Comamonas, C. testosteroni ha sido la especie más frecuentemente reportada como causa de infecciones en humanos, describiéndose solo 23 casos de C. kerstersii. Dado los problemas en la identificación fenotípica de estos microorganismos, es posible que algunos de los reportes de C. testosteroni pudieran haber correspondido a C. kerstersii. Este caso pone en evidencia el rol patógeno de C. kerstersii y la importancia de utilizar MALDI-TOF como herramienta diagnóstica en bacilos gramnegativos no fermentadores.
Abstract Comamonas kerstersii is a non-fermenting Gram-negative bacillus. It has been associated with intra-abdominal infections. We describe a clinical case of bacteremia caused by C. kerstersii in a young man with acute appendicitis. The first identification, using the Vitek2 compact system (BioMerieux) from blood cultures, was Comamonas testosteroni. However, using MALDI-TOF was identified as C. kerstersii. The patient was treated first with ampicillin sulbactam and then piperacillin tazobactam with favorable evolution. Within the genus Comamonas, C. testosteroni has been the most frequently reported species as a cause of infections in humans, only 23 cases of C. kerstersii being described. Given the problems of phenotypic identification of these microorganisms, it is possible that some C. testosteroni reports could have corresponded to C. kerstersii. This case highlights the pathogenic role of C. kerstersii and the importance of using MALDI-TOF as a diagnostic tool for non-fermenting Gram-negative bacilli identification.
Subject(s)
Humans , Male , Appendicitis , Bacteremia , Comamonas , Gram-Negative BacteriaABSTRACT
Comamonas testosteroni and Acinetobacter guillouiae are gram-negative bacilli of low virulence that are widely distributed in nature and normal flora. Despite their common occurrence in environments, they rarely cause infectious disease. We experienced a case of septic shock by C. testosterone and A. guillouiae, and isolated them by 16S ribosomal RNA sequencing method from the blood cultures of a previous healthy female during postoperative supportive care. This is the first case of septic shock required ventilator care and continuous renal replacement therapy due to these organisms in Korea.
Subject(s)
Female , Humans , Acinetobacter , Bacteremia , Comamonas testosteroni , Comamonas , Communicable Diseases , Korea , Renal Replacement Therapy , RNA, Ribosomal, 16S , Shock, Septic , Testosterone , Ventilators, Mechanical , VirulenceABSTRACT
Comamonas testosteroni and Acinetobacter guillouiae are gram-negative bacilli of low virulence that are widely distributed in nature and normal flora. Despite their common occurrence in environments, they rarely cause infectious disease. We experienced a case of septic shock by C. testosterone and A. guillouiae, and isolated them by 16S ribosomal RNA sequencing method from the blood cultures of a previous healthy female during postoperative supportive care. This is the first case of septic shock required ventilator care and continuous renal replacement therapy due to these organisms in Korea.
Subject(s)
Female , Humans , Acinetobacter , Bacteremia , Comamonas testosteroni , Comamonas , Communicable Diseases , Korea , Renal Replacement Therapy , RNA, Ribosomal, 16S , Shock, Septic , Testosterone , Ventilators, Mechanical , VirulenceABSTRACT
An aquifer located within a petroleum processing plant in Moin, Costa Rica, suffers hydrocarbon pollution. This study aimed to determine the ability of indigenous microorganisms from this site to degrade polycyclic aromatic hydrocarbons [PAHs] to evaluate the feasibility of an eventual bioremediation process. Aerobic conditions were found in the aquifer, while microbial analyses of the groundwater indicated the presence of important hydrocarbon-degrading populations. Sixteen PAH-degrading strains were isolated with the ability to grow on naphthalene [5 strains], phenanthrene [3], fluorene [6] and pyrene [2]. Most of the identified isolates belonged to the genus Pseudomonas, although, Comamonas, Sphingomonas Stenotrophomonas and Delftia were also found. A mixture of selected strains was evaluated by its performance of PAH degradation in soil-slurry systems, where efficiency of removal was naphthalene > fluorene > phenanthrene > pyrene. This study is an initial approach to evaluate the feasibility of applying a bioremediation process in the contaminated site
Subject(s)
Biodegradation, Environmental , Petroleum , Environmental Pollution , Polycyclic Aromatic Hydrocarbons , Groundwater , Naphthalenes , Phenanthrenes , Fluorenes , Pyrenes , Pseudomonas , Comamonas , Sphingomonas , Stenotrophomonas , DelftiaABSTRACT
Comamonas testosteroni has rarely been implicated as a human pathogen. We here present a case of peritonitis due to this organism in a patient on continuous ambulatory peritoneal dialysis (CAPD). A 32-year-old woman was admitted with abdominal pain and cloudy peritoneal effluent. Empirical intraperitoneal (IP) treatment with cefazolin and ceftazidime was started. The culture was positive for C. testosteroni and antibiotic was changed to ceftazidime IP. Four days after the ceftazidime treatment, the patient became asymptomatic. The follow-up culture from peritoneal effluent at 7th day was negative. This treatment was maintained for 21 days. After that, culture negative peritonitis occurred twice for 2 months in this patient, so CAPD catheter had to be removed. This is the first reported case of CAPD peritonitis caused by C. testosteroni. It is important for clinicians to recognize that CAPD peritonitis is caused by this organism which has been largely overlooked as a potential pathogen.
Subject(s)
Adult , Female , Humans , Abdominal Pain , Catheters , Cefazolin , Ceftazidime , Comamonas , Comamonas testosteroni , Follow-Up Studies , Peritoneal Dialysis , Peritoneal Dialysis, Continuous Ambulatory , PeritonitisABSTRACT
PURPOSE: To report a case of a Comamonas acidovarans corneal ulcer treated with antibiotics in Korea. Comamonas acidovarans corneal ulcer has not been previously reported to our knowledge. METHODS: A 70-year-old woman visited our clinic complaining of left ocular pain and decreased visual acuity for six days. We performed a smear and culture on the corneal lesion and treated it with topical and systemic antibiotics. RESULTS: Comamonas acidovorans was cultured from ulcer lesion. Corneal ulcer improved with the administration of ciprofloxacin and tobramycin.
Subject(s)
Aged , Female , Humans , Anti-Bacterial Agents , Ciprofloxacin , Comamonas , Corneal Ulcer , Delftia acidovorans , Korea , Tobramycin , Ulcer , Visual AcuityABSTRACT
To date, only one case of peritonitis with exit site infection in peritoneal dialysis caused by this micro- organism has been reported. In spite of its apparently benign clinical course, which distinguished it from peritonitis caused by Pseudomonas, this peritonitis relapsed and Comamonas could not be eliminated from the peritoneal liquid, probably due to the persistence of the micro-organism in the exit site. Consequently, peritoneal catheter was removed. In this case, a 68-year-old man was admitted with fever, abdominal tenderness and cloudy peritoneal effluent and empirically treated with antibiotics(cefazolin, tobramycin), intraperitoneally(IP) for 7 days. The first culture was positive for Comamonas acidovorans, sensitive to ceftazidime, cefotetan, ceftriaxone, ciprofloxaxin and imipenem and the perotoneal effluent remained cloudy after 7 days. He was treated with ceftazidime IP, oral ciprofloxacin and nystatin for 26 days. 4 days after the antibiotics treatment, the patient was asymptomatic and the cell count of peritoneal effluent was 50 WBC/mm3 with negative culture. 25 days after the treatment, the patient remained asymptomatic and with 5 WBC/mm3 in peritoneal effluent. Consequently, We experienced a case of peritonitis due to Comamonas acidovorans in a patient on CAPD without exit site infection and managed with preservation of the catheter.